Molecular biology research demands precision, and that's exactly what you get with PowerPCR Pfu DNA polymerase. This highly thermostable enzyme derived from the hyperthermophilic archaeum Pyrococcus furiosus brings exceptional accuracy to your PCR experiments, ensuring your results are both reliable and reproducible.
What sets Pfu DNA polymerase apart is its remarkable 6-10 fold higher fidelity compared to traditional Taq DNA polymerase. This superior accuracy stems from its inherent 3' to 5' exonuclease proofreading activity, which detects and corrects errors during DNA synthesis. For researchers working with cloning, expression studies, or cDNA applications, this level of precision isn't just beneficial—it's essential for success.
Durability meets performance with this enzyme's impressive thermostability. Pfu DNA polymerase maintains 95% of its activity even after 2 hours at 95°C, making it perfect for demanding PCR protocols. With an elongation velocity of 0.2-0.4 kb/min at 70-75°C, it efficiently amplifies DNA targets up to 2 kb, giving you flexibility in experimental design.
Beyond standard PCR, this enzyme excels in specialized applications. It readily incorporates modified nucleotides including biotin-, digoxigenin-, and fluorescently-labeled versions, expanding your detection options. The generation of blunt-ended PCR products simplifies downstream cloning procedures, saving you time and improving cloning efficiency.
Whether you're performing high-fidelity PCR, generating products for cloning and expression, conducting RT-PCR for cDNA work, or performing blunt-end PCR cloning, PowerPCR Pfu DNA polymerase delivers the performance and reliability your research demands. Each 1,000 unit vial contains 2.5 U/µl concentration, providing ample enzyme for multiple experiments and consistent results across your lab's workflows.
